Coding
ARS B2-B2
Part:BBa_K2984000:Design
Designed by: Sophia Schiebler Group: iGEM19_Humboldt_Berlin (2019-10-02)
3'UTR, Secretion signal of Arylsulfatase ARS B2-B2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This was translated to fit the codon usage of C. reinhardtii.
Source
It is an endogenous secretion signal of C. reinhardtii's nuclear genome.
References
Rasala, B. A., Lee, P. A., Shen, Z., Briggs, S. P., Mendez, M., & Mayfield, S. P. (2012). Robust Expression and Secretion of Xylanase1 in Chlamydomonas reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide. PLOS ONE, 7(8), e43349. Retrieved from https://doi.org/10.1371/journal.pone.0043349