Regulatory

Part:BBa_K2992041

Designed by: Yaseen Tengur   Group: iGEM19_Nottingham   (2019-10-01)
Revision as of 10:41, 1 October 2019 by YasTen (Talk | contribs)


5-UTR containing RBS for ha33 gene from C. botulinum

5-UTR containing RBS for ha33 gene from C. botulinum.

Usage and Biology

This parts entry represents the 5’-UTR containing the RBS for the hemagglutinin component of the botulinum neurotoxin complex from C. botulinum. Since ha33 is natural constituent of the BotR regulon, we chose this promoter to control the expression of our FAST reporter construct thus linking reporter fluorescence with botulinum neurotoxin production. In doing so, we hoped to generate our surrogate host strain as a model for predicting neurotoxin production in foodstuffs following food manufacturing processes.

Characterisation

Data incoming.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Dupuy and Matamouros, 2006 Raffestin et al 2009 update


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Categories
Parameters
None