Part:BBa_K2992041

5-UTR containing RBS for ha33 gene from C. botulinum

5-UTR containing RBS for ha33 gene from C. botulinum.

Usage and Biology

This parts entry represents the 5’-UTR containing the RBS for the hemagglutinin component of the botulinum neurotoxin complex from C. botulinum. Since ha33 is natural constituent of the BotR regulon, we chose this promoter to control the expression of our FAST reporter construct thus linking reporter fluorescence with botulinum neurotoxin production. In doing so, we hoped to generate our surrogate host strain as a model for predicting neurotoxin production in foodstuffs following food manufacturing processes.

Characterisation

Data incoming.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Dupuy, B. et al., 2006. Regulation of toxin and bacteriocin gene expression in Clostridium by interchangeable RNA polymerase sigma factors. Molecular Microbiology, 60(4), pp.1044–1057.

Raffestin, S., Dupuy, B., Marvaud, J. and Popoff, M. (2004). BotR/A and TetR are alternative RNA polymerase sigma factors controlling the expression of the neurotoxin and associated protein genes in Clostridium botulinum type A and Clostridium tetani. Molecular Microbiology, 55(1), pp.235-249.