Regulatory
RnaG120

Part:BBa_K2588050

Designed by: Bjarne Klopprogge, Daniel Wedemeyer   Group: iGEM18_Hamburg   (2018-10-09)
Revision as of 03:55, 18 October 2018 by BKlopprogge (Talk | contribs)

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RnaG120-based NOT gate to invert promotor function

Using the terminator BBa_B0015, the constitutive promotor BBa_J23106, and the RnaG120 based terminator BBa_K2588004 the function of a promotor cloned downstream of this sequence in reverse direction can invert the promotors function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
    Illegal NheI site found at 167
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None