Part:BBa_K2656201

Briobrickation part

This part is a transcriptional unit including:

• BBa_K2656004 (GoldenBraid 3.0. version of J23106 promoter)
• BBa_K2656009 (GoldenBraid 3.0. version of B0030 RBS)
• BBa_K2656014 (GoldenBraid 3.0. version of E1010 CDS, mRFP1)
• BBa_K2656026 (GoldenBraid 3.0. version of B0015 terminator)

In addition, this part is flanked upstream with the sequence ggagtgagacg and downstream with the sequence cgtctcagtca. These sequences include a BsmbI Type IIS endonuclease recognition site and the appropriate resulting sticky ends to accept a GoldenBraid 3.0 alpha1 insert.

This construction has been ligated into the BB backbone pSB1C3, so designing the pBioBrickator plasmid. Thus, in this BB compatible vector, BBa_K2656201 acts as a selection marker to carry out the red-white screening (Figure 1) while subcloning a composite GoldenBraid construction into the pSB1C3 vector. Moreover, this selection marker part can also be subcloned into any other Biobrick compatible backbone to give the new plasmid the same ability to accept GoldenBraid transcriptional units.

Figure 1. Example of Biobrickation reaction and red-white(green in this case) selection.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 18
  Illegal NheI site found at 41
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 627
  Illegal AgeI site found at 739
  
• 1000
  COMPATIBLE WITH RFC[1000]