Cell

Part:BBa_K2841503

Designed by: Jack Lawrence, Rhys Evans, Jonny Whiteside   Group: iGEM18_Warwick   (2018-10-12)
Revision as of 20:57, 17 October 2018 by RhysEvans97 (Talk | contribs)


Legionella Hemolysin Detector

A dCAS9 (BBa_K2841510) gRNA (BBa_K2841514) capable of repressing the LacIq promoter (BBa_K2841517) in response to [http://2018.igem.org/Team:Warwick/Legiolysin Legionella Hemolysin] encoding mRNA. Recognises the Alpha (BBa_K2841531) imitating motif.

This part shows 2.1-2.5-fold upregulation upon induction of activity.

T--Warwick--2018-Alpha-Graphs.png

Raw Data

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 145
    Illegal NgoMIV site found at 174
    Illegal NgoMIV site found at 408
    Illegal NgoMIV site found at 437
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None