Part:BBa_E0430

EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)

Standard YFP Output Device -LVA tag

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Team USP-Brazil 2018 Improvement

Team USP-Brazil, in 2018, has improved this part (BBa_K2771020) by adding it to a control gene, being that CFP constitutively expressed by ptet, which serves as a proxy for external influences on the first reporter's measurement, such as cell density, plasmid copy number and ,to some amount, metabolic burden:

File:File:T--USP-Brazil--ratiometric.png

Figure 1: Scheme of USP-Brazil's improvement BBa_K2771020