RNA

Part:BBa_K2611004

Designed by: Yuanhan Yang   Group: iGEM18_SCU-China   (2018-10-17)
Revision as of 07:19, 17 October 2018 by Yangyuanhan (Talk | contribs)


sgRNA (J23100)

We designed this sgRNA that are complementary to the J23100 constitutive promoter using a special algorithm. This sgRNA can direct dCas9 protein to combine with the J23100 constitutive promoter to block the transcription. As a result, the expression of the downstream will decrease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None