RNA

Part:BBa_K2611004

Designed by: Yuanhan Yang   Group: iGEM18_SCU-China   (2018-10-17)


sgRNA (J23100)

We designed this sgRNA that are complementary to the J23100 constitutive promoter by BLAST all spacer sequence generated by GFP open reading frame with the genome of E. coli BL21 strain. This sgRNA can direct dCas9 protein to combine with the J23100 constitutive promoter to block the transcription. As a result, the expression of the downstream will decrease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None