Composite
Toehold S
Part:BBa_K2550000:Design
Designed by: Janet Standeven, Abby Bell, Megan Hong, Yan Zhang, Courtney Taing, Ashtyn Cauffman Group: iGEM18_Lambert_GA (2018-10-08)
T7 promoter Toehold Ribosome Switch with LacZ expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Needs to be used in low to medium copy plasmids only
Source
References
Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin, Toehold Switches: De-Novo-Designed Regulators of Gene Expression, Cell, Volume 159, Issue 4, 2014, Pages 925-939, ISSN 0092-8674, https://doi.org/10.1016/j.cell.2014.10.002