Composite
Toehold S

Part:BBa_K2550000:Design

Designed by: Janet Standeven, Abby Bell, Megan Hong, Yan Zhang, Courtney Taing, Ashtyn Cauffman   Group: iGEM18_Lambert_GA   (2018-10-08)
Revision as of 15:28, 15 October 2018 by ABell (Talk | contribs)


T7 promoter Toehold Ribosome Switch with LacZ expression



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Needs to be used in low to medium copy plasmids only

Source

References

Alexander A. Green, Pamela A. Silver, James J. Collins, Peng Yin, Toehold Switches: De-Novo-Designed Regulators of Gene Expression, Cell, Volume 159, Issue 4, 2014, Pages 925-939, ISSN 0092-8674, https://doi.org/10.1016/j.cell.2014.10.002