Coding
SAMT

Part:BBa_J45005:Design

Designed by: Kate Broadbent   Group: iGEM06_MIT   (2006-06-07)
Revision as of 22:01, 9 March 2008 by Rshetty (Talk | contribs)

SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winterg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 652
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 232

Design Notes

  1. No BioBrick restriction enzyme cut sites in coding region
  2. Changed natural stop codon to TAA TAA
  3. No template DNA available and thus could not construct by PCR

Source

SAMT from Clarkia breweri ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=6002711 GenBank AF133053])

References

<biblio>

  1. Ross-ArchBiochemBiophys-1999 pmid=10375393

</biblio>

  • [http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 MIT iGEM 2006 notebook entry]