Part:BBa_J45003

SAM:jasmonic acid carboxyl methyltransferase; converts jasmonic acid to jasmine odor

This ORF encodes the enzyme responsible for catalyzing the following reaction:

Jasmonic Acid--->Methyl Jasmonate

It is initially found in Arabidopsis thaliana but has been transferred successfully to the E. coli genome by Seo, et al. The product, methyl jasmonate has a jasmine smell to it. For more information, see http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6.

Usage and Biology

• 1 mM jasmonic acid and 1 mM S-adenosyl methionine (SAM)
• Seo et al. were able to get the enzyme, purified from E. coli, to catalyze the jasmine-smelling reaction in vitro.
• Km: 38.5 μM; Kcat:25 S−1

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 717
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1162
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 613