1 Registry Star
Generator
Banana
Part:BBa_J45200:Experience
Designed by: André Green II Group: iGEM06_MIT (2006-10-20)
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Applications of BBa_J45200
The production of isoamyl acetate was verified by scent: E. coli with no BSGD did not produce isoamyl acetate (that is, they did give off banana scent) when isoamyl alcohol was added to the medium, while E. coli with the BSGD did produce isoamyl acetate when isoamyl alcohol was added to the medium.
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UNIQ7aac7aec01f81be8-partinfo-00000000-QINU UNIQ7aac7aec01f81be8-partinfo-00000001-QINU
Characterization
Transcriptional control of banana odor enzyme generator
Growth dependent banana odor production
To demonstrate growth phase dependent banana odor production, we compared the behavior of constitutive and stationary phase dependent banana odor generators (A and B, respectively). We measured isoamyl acetate concentrations of cultures of the constitutive and stationary phase banana odor generators at different culture cell densities (OD600nm) (C). As expected, the stationary phase banana odor generator produced very little isoamyl acetate at low cell densities but its isoamyl acetate production increased with cell density. By comparison, the constitutive banana odor generator produced more isoamyl acetate at lower cell densities than the stationary phase banana odor generator. To visually aid comparison of the two odor generators, an empirical fit to the data for each device is shown.
To demonstrate growth phase dependent banana odor production, we compared the behavior of constitutive and stationary phase dependent banana odor generators (A and B, respectively). We measured isoamyl acetate concentrations of cultures of the constitutive and stationary phase banana odor generators at different culture cell densities (OD600nm) (C). As expected, the stationary phase banana odor generator produced very little isoamyl acetate at low cell densities but its isoamyl acetate production increased with cell density. By comparison, the constitutive banana odor generator produced more isoamyl acetate at lower cell densities than the stationary phase banana odor generator. To visually aid comparison of the two odor generators, an empirical fit to the data for each device is shown.