Regulatory
p(osmY)

Part:BBa_J45993:Design

Designed by: Stephen Payne   Group: iGEM06_MIT   (2006-07-11)
Revision as of 23:38, 7 March 2008 by Rshetty (Talk | contribs)

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Minimal stationary phase osmY promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Constructed by primer annealing and extension

Template

None.

Forward Primer

5'- CTA GAG GCT TAT GTT TTC GCT GAT ATC CCG AGC GGT TTC AAA ATT GTG ATC TAT ATT TAA CAA ATA CTA GTA GCG GCC GCT GCA -3'

Reverse Primer

5'- GCG GCC GCT ACT AGT ATT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG CTC GGG ATA TCA GCG AAA ACA TAA GCC T -3'

Source

  • Promoter controlling expression of osmY from Escherichia coli (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278])

References

<biblio>

  1. Yim-1994 pmid=8282684
  2. Schellhorn-1998 pmid=9829938
  3. Wise pmid=8631665
  4. Funabashi pmid=11879714
  5. Makinoshima pmid=11985708
  6. Yim-1992 pmid=1317380
  7. Hengge-Aronis-1993 pmid=8416901
  8. Vijaykumar-2004 pmid=15576800

</biblio>


[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.