Part:BBa_J45992:Design

Full-length stationary phase osmY promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Primers used to PCR this part out of the E.coli genome:

Forward Primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCT GGC ACA GGA ACG TTA TC - 3'

Reverse Primer

5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG- 3'

Source

This part was PCRed out of E. coli's genome with the primers listed in the design notes section.

References

<biblio>

1. Yim-1994 pmid=8282684
2. Schellhorn-1998 pmid=9829938
3. Wise pmid=8631665
4. Funabashi pmid=11879714
5. Makinoshima pmid=11985708
6. Yim-1992 pmid=1317380
7. Hengge-Aronis-1993 pmid=8416901
8. Vijaykumar-2004 pmid=15576800

</biblio>

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.