Coding
THI3

Part:BBa_J45009

Designed by: Veena Venkatachalam   Group: iGEM06_MIT   (2006-06-07)
Revision as of 22:43, 7 March 2008 by Rshetty (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal

BBa_J45009 encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1.

Usage and Biology

  • THI3 is formerly known as KID1.
  • 3-methylbutanal is reduced via a chassis alcohol dehydrogenase to isoamyl alcohol which is a precursor to isoamyl acetate (banana odor).
  • Note: BBa_J45009 has several internal BioBrick restriction sites. We indicate proposed silent mutations for eliminating the BioBrick restriction sites. It can only be used as a suffix part in BioBrick assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/odor
s
Parameters
ec_num
kegg
proteinTHI3
swisspro