Coding
TEVpN

Part:BBa_K2549014

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-05)
Revision as of 04:46, 7 October 2018 by Wave (Talk | contribs)


TEV protease N-terminal fragment

This part is N-terminal fragment of TEVp (Part:BBa_K2549013). TEV protease is one of the best-characterized enzymes of the viral proteases which have more stringent sequence specificity. In out project, TEVpN was fused to CfaN (Part:BBa_K2549009) intein, when co-expressed with CfaC (Part:BBa_K2549010) + TEVpC (Part:BBa_K2549015) in the same cell, will reconstitute site-specific protease activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316

Usage and Biology

TBA



References

[edit]
Categories
//cds/enzyme/protease
//function/degradation
Parameters
None