Part:BBa_J45014:Design

alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 99
  Illegal BamHI site found at 1426
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 526
  Illegal SapI.rc site found at 1534
  

Design Notes

• BBa_J45014 differs from BBa_J45006 by a single point mutation at base pair 1148 (T -> C).

New forward primer

5' - GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAA TGA AAT CGA TGA GAA AAA TC -3'

New reverse primer

5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAa ggg cct aaa agg aga gct ttg -3'

Source

ATF1 gene in Saccharomyces cerevisiae.

References

<biblio>

1. Horton03 pmid=12937998
2. Singh--2007 pmid=17891501

</biblio>