Coding
ATF1

Part:BBa_J45014:Design

Designed by: André Green II   Group: iGEM06_MIT   (2006-07-31)
Revision as of 15:49, 5 February 2008 by Rshetty (Talk | contribs)

alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534


Design Notes

  • This part differs from ATF1 BBa_J45006 at one base pair, (1148; T -> C).
  • It is currently in the pSB1A3-1 BioBrick backbone.
  • new forward primer: 5' - GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAA TGA AAT CGA TGA GAA AAA TC -3'
  • new reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAa ggg cct aaa agg aga gct ttg -3'

Source

This part comes from BBa_J45006.

References

<biblio>

  1. Horton03 pmid=12937998
  2. Singh--2007 pmid=17891501

</biblio>