Coding
ATF1
Part:BBa_J45014
Designed by: André Green II Group: iGEM06_MIT (2006-07-31)
alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)
This part is the ATF1 sequence BBa_J45006 with a single point mutation at base pair 1148 (T -> C). Alcohol acetyltransferase I (ATFI) encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols. It was obtained from S. cerevisiae.
Usage and Biology
[Note that this is not aspartate amino transferase (also called "AATase"), and that "ATF1" also refers to "Activating Transcription Factor 1" in humans (an entirely different protein that is homologous to "Atf1" in mouse).]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 99
Illegal BamHI site found at 1426 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 526
Illegal SapI.rc site found at 1534
[edit]
Categories
Parameters
//function/biosynthesis/odorant
//function/odor
s
//function/odor
s
ec_num | 2.3.1.84 |
kegg | sce:YOR377W |
protein | ATF1-1148 mutant |
swisspro | P40353 |