Composite

Part:BBa_K2669003:Design

Designed by: Matilda Brink   Group: iGEM18_Uppsala   (2018-10-02)
Revision as of 10:39, 2 October 2018 by MatildaBrink (Talk | contribs)


Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite part is equipped with primer binding sites for VF2 and VR, prefix and suffix and contains Biobrick scar sequence.


Source

RBS (BBa_J34801), constitutive promotor (BBa_J23119), optimized AmilCP (BBa_K2669002) and double terminator (BBa_B0014), primer binding sites VF2 and VR (BBa_G00100), (BBa_G00102), prefix and suffix in pbs1c3 backbone.

References