Composite

Part:BBa_K2669003:Design

Designed by: Matilda Brink   Group: iGEM18_Uppsala   (2018-10-02)


Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The sequence was codon optimized with the codon optimization tool provided by IDT.

Biobrick was ordered as a customized genes from Integrated DNA Technologies (IDT)

constitutive promotor (BBa_J23119), RBS (BBa_J34801), codon optimized original native AmilCP (BBa_K2669002) and double terminator (BBa_B0014).

References