Composite
Part:BBa_K2669003:Design
Designed by: Matilda Brink Group: iGEM18_Uppsala (2018-10-02)
Strong constitutive promotor + RBS + Optimized Amil CP (BBa_K2669002) + double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The composite part is equipped with primer binding sites for VF2 and VR and contains Biobrick scar sequence.
Source
RBS (BBa_J34801), constitutive promotor (BBa_J23119), optimized AmilCP (BBa_K2669002) and double terminator (BBa_B0014).