Part:BBa_K2810007

siRNA from 5' region of Myzus persicae SP3 secreted protein

SP3 is a predicted secreted protein that localizes to [http://rspb.royalsocietypublishing.org/content/280/1750/20121952 aphids bacteriocyte cells].

This sequence is taken from the complement strand from a 5’ region of the Myzus persicae SP3 gene. The Cardiff_Wales team is attempting to target this gene using RNAi.

SP3_5_3 is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI or Esp3I and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard].

It is flanked by BsaI sites together 5' AATG and 3' GCTT cloning sequences for subsequent cloning into a level 1 plasmid.

This sequence is incorporated into a level 1 plasmid for plant expression as part of an RNAi construct with the coding sequence found in BBa_K2810005.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]