Regulatory

Part:BBa_K2810000

Designed by: Geraint Parry   Group: iGEM18_Cardiff_Wales   (2018-08-24)
Revision as of 17:10, 24 August 2018 by Gparry75 (Talk | contribs) (Created page with "__NOTOC__ <partinfo>BBa_K2810000 short</partinfo> This part contains the sequence of the WRKYintron, which is commonly used as to separate the sense and anti-sense parts of a...")

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WRKY intron for plant-targeted RNAi constructs

This part contains the sequence of the WRKYintron, which is commonly used as to separate the sense and anti-sense parts of an RNAi contruct for gene silencing. This has been used many times by plant biologists and is part of the pJawohl8-RNAi binary plasmid.

The WRKYintron is cloned into the the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard. It is flanked by BsaI sites together 5' CCAT and 3' AATG cloning sequences for subsequent cloning into a level 1 plasmid.

This has been used to generate the RNAi constructs detailed in the 2018 Cardiff_Wales Parts page

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None