Measurement

Part:BBa_J100431

Designed by: Tatianna Travieso   Group: Campbell M Lab   (2018-06-28)
Revision as of 17:50, 28 June 2018 by Registry (Talk | contribs)

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Modified T7 Promoter with Gal4 Binding Site in pClone mScarlet

Modified T7 promoter (J100404) in which "TAATAC" has been removed and the 17 base pair sequence of the Gal4 binding site has replaced it inserted into pClone mScarlet (J100397). This encodes for RFP when inserted in into pClone. When present the Gal4 protein will bind to this site.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 906
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 906
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 906
    Illegal BamHI site found at 852
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 906
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 906
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None