Measurement

Part:BBa_J100431

Designed by: Tatianna Travieso   Group: Campbell M Lab   (2018-06-28)


Modified T7 Promoter with Gal4 Binding Site in pClone mScarlet

Modified T7 promoter (J100404) in which "TAATAC" has been removed and the 17 base pair sequence of the Gal4 binding site has replaced it inserted into pClone mScarlet (J100397). This encodes for mScarlet when inserted in into pClone mScarlet. When present the Gal4 protein will bind to this site.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 906
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 906
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 906
    Illegal BamHI site found at 852
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 906
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 906
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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