Composite

Part:BBa_J100414

Designed by: Caroline Willis, Tatianna Travieso   Group: Campbell M Lab   (2018-06-21)
Revision as of 14:16, 26 June 2018 by Tatravieso (Talk | contribs)


T7 Promoter with Downstream Gal4 Binding Site in pClone Red

Gal4 binding site inserted downstream of a T7 promoter consensus sequence (J100403) that will produce RFP when inserted in pClone Red (J119137) . When present the Gal4 protein will bind to the site downstream of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1454
    Illegal AgeI site found at 1566
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None