Composite

Part:BBa_J100414

Designed by: Caroline Willis, Tatianna Travieso   Group: Campbell M Lab   (2018-06-21)


T7 Promoter with Downstream Gal4 Binding Site in pClone Red

Gal4 binding site inserted downstream of a T7 promoter consensus sequence (J100403) that will produce RFP when inserted into pClone Red (J119137) . When present the Gal4 protein will bind to the site downstream of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1454
    Illegal AgeI site found at 1566
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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