Composite

Part:BBa_M50437

Designed by: Lillian Zhu, Kelsie Wysong, Eva Frankel   Group: Stanford BIOE44 - S11   (2018-04-25)
Revision as of 22:33, 11 June 2018 by Lilzhu8 (Talk | contribs)


2,3-DHB Biosynthesis Construct, with entC and ahpC

This device codes for enzymes that are part of the biosynthetic pathway of enterobactin, a compound with a strong affinity for iron (III) ions, which are naturally produced by bacteria to sequester this essential trace nutrient. The specific aim of this construct is increasing the production of 2,3-DHB, an intermediate that is itself a strong coordinator to Fe (III) ions. Upregulating production of this small molecule should grant bacteria the ability to increase dissolution of insoluble iron (III) compounds like iron (III) oxide.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 234
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 234
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 234
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None