![](https://parts.igem.org/images/partbypart/icon_composite.png)
Part:BBa_K2442405
[split GFP module 1] tetR repressible promoter + RBS + GFP N-term + double terminator
This is a composite of the GFP N-term split subunit (GFP1) K1789003, the well characterised double-terminator construct B0015, and the well characterised tetR regulated promoter R0040
This N-term GFP part was found to contain an extra A nucleotide immediately preceding the start-codon ATG. This erroneous nucleotide is present in the parts-registry sequencing for K1789003, but is not reported as an error. Unfortunately, now both split-GFP modules might be affected by improperly spaced ribosome binding sites. Despite this we proceeded to the next assembly stage.
![](/wiki/images/8/81/Glasgow_ANDgate_Figure_4.png)
Usage and Biology
this composite was used for assembly of larger composite split-GFP AND-gate constructs.
- K2442408 Full split-GFP AND-gate circuit [Split GFP module 1 + Split GFP module 2]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |