Coding

Part:BBa_K2457003:Design

Designed by: Maria Astolfi, Giovanna Maklouf, Juliana Vitoriano   Group: iGEM17_Amazonas_Brazil   (2017-07-09)
Revision as of 01:35, 2 November 2017 by JulianaNV (Talk | contribs) (→‎Design Notes)


Standardized RecA coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 508
    Illegal AgeI site found at 1012
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is, 1130 bp of lenght, based on Escherichia coli strain MG1655 (Zhao 2016). This RecA is E. coli codon optimized, by changing codons from EcoRI and NotI sites in the codifying sequence of RecA, without removing the phase gene.

Source

Source

References