Software error:
Can't call method "errstr" on an undefined value at /websites/parts.igem.org/cgi/lib/BBDB.pm line 35. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Plate.pm line 13. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Plate.pm line 13. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Well.pm line 11. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Well.pm line 11. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Blast.pm line 15. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Blast.pm line 15. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Sample.pm line 24. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Sample.pm line 24. Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10. Compilation failed in require at /websites/parts.igem.org/cgi/extensions/wiki_header_hook.cgi line 9. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/extensions/wiki_header_hook.cgi line 9.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Status: 500
Content-type: text/html
Software error:
Can't call method "errstr" on an undefined value at /websites/parts.igem.org/cgi/lib/BBDB.pm line 35. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Plate.pm line 13. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Plate.pm line 13. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Well.pm line 11. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Well.pm line 11. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Blast.pm line 15. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Blast.pm line 15. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Sample.pm line 24. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Sample.pm line 24. Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10. Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Status: 500
Content-type: text/html
Software error:
Can't call method "errstr" on an undefined value at /websites/parts.igem.org/cgi/lib/BBDB.pm line 35. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Plate.pm line 13. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Plate.pm line 13. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Well.pm line 11. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Well.pm line 11. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Blast.pm line 15. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Blast.pm line 15. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Sample.pm line 24. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Sample.pm line 24. Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10. Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Design Notes
This sequence is, 1130 bp of lenght, based on Escherichia coli strain MG1655 (Zhao 2016). This RecA is E. coli codon optimized, by changing codons from EcoRI and NotI sites in the codifying sequence of RecA, without removing the phase gene.
References
Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald L. Court. An efficient recombination system for chromosome engineering in Escherichia coli. PNAS 2000 97 (11) 5978-5983; May 16, 2000
GENETIC ENGINEERING USING HOMOLOGOUS RECOMBINATION1 Donald L. Court, James A. Sawitzke, and Lynn C. Thomason. Annu. Rev. Genet. 2002. 36:361–88
Dongdong Zhao, Shenli Yuan, Bin Xiong, Hongnian Sun, Lijun Ye, Jing Li, Xueli Zhang and Changhao Bi .Development of a fast and easy method for Escherichia coli genome editing with CRISPR/ Cas9. 2016.