Reporter

Part:BBa_K2486010:Design

Designed by: Cau Westmann and Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-24)
Revision as of 00:29, 2 November 2017 by Gabriellovate (Talk | contribs) (→‎References)


PlldR_tetR reporter circuit (GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 100
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 810


Design Notes

the lva tag at the gfp is suitable for knetic analysis of promoters


Source

synthesis

References

Lutz, R., & Bujard, H. (1997). Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic acids research, 25(6), 1203-1210.