Reporter

Part:BBa_K2486010:Design

Designed by: Cau� Westmann and Gabriel Lencioni Lovate   Group: iGEM17_USP-Brazil   (2017-10-24)


PlldR_tetR reporter circuit (GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 100
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 810


Design Notes

We developed a new hybrid promoter that responds to LldR and TetR, which possiblites the design of and gates for lactate and tetracycline or generation of other combinations trough cascade expression of genes. We adopted the TetR operator because of its tight regulation, which reduces basal signal in biosensors.

Source

This part was generated through synthesis and posterior inserion in pSB1C3 through 3A assembly.

References

  1. Lutz, R., & Bujard, H. (1997). Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic acids research, 25(6), 1203-1210.
  2. Andersen, Jens Bo, et al. "New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria." Applied and environmental microbiology 64.6 (1998): 2240-2246.