Part:BBa_I13521

Ptet mRFP

Constitutive on mRFP (positive control)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 635
  Illegal AgeI site found at 747
  
• 1000
  COMPATIBLE WITH RFC[1000]

Pictures

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  BBa_I13521 visualized under a non-UV lightbox

• 

  BBa_I13521 visualized under 254nm wavelength UV lightbox

The followings were edited by iGEM17_SCU-China

Improvement

We altered the RBS region and constructed new parts that has higher translation rate. To test the performance, we measure the fluorescence intensity and OD600 changes of the bacteria liquid over time within 16hours after inoculated. The result showed that we indeed improved the Part:BBa_I13521 and got a new part, BBa_K2276010, containing a stronger RBS. Fluorescence quenching may explain the drop of the fluorescence intensity of P3 strain at 16h because the concentration of fluorescent protein was too high.

Fig 1.The fluorescence intensity changes over time (0h~16h).P1 represent the strain containing part: BBa_K2276007.P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.

Fig 2.The OD600 changes over time (0h~16h).P1 represent the strain containing part: BBa_K2276007.P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.