Part:BBa_K525998

Promoter T7 and RBS

T7 promoter and RBS. The T7 promoter does not work with the RNA polymerase from Escherichia coli but with the RNA polymerase from the T7 phage. To express BioBricks under the control of a T7 promoter, E. coli carrying a T7 polymerase gene have to be used (e.g. BL21(DE3) or KRX).

Used to express e.g. BBa_K525123 - look there for typical induction profiles.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Improvement of K525998 by ZJU-CHINA 2017 Teams

The ZJU-CHINA 2017 Teams fulfilled the improvement of K525998 by inducing some mutation in the sequence. A series of promoters with different expression strength are constructed. The result can be seen by clicking the link below.

<a href=https://parts.igem.org/Part:BBa_K2207024>BBa_K2207024</a>

<a href=https://parts.igem.org/Part:BBa_K2207025>BBa_K2207025</a>

<a href=https://parts.igem.org/Part:BBa_K2207026>BBa_K2207026</a>

<a href=https://parts.igem.org/Part:BBa_K2207027>BBa_K2207027</a>

<a href=https://parts.igem.org/Part:BBa_K2207028>BBa_K2207028</a>

<a href=https://parts.igem.org/Part:BBa_K2207029>BBa_K2207029</a>

<a href=https://parts.igem.org/Part:BBa_K2207030>BBa_K2207030</a>

Contribution2

Part name:BBa_K2382013
Group: iGEM17_CSMU_NCHU_Taiwan 2017
Author: SHAO-CHI LO
Summary: We add a His Tag at the end. Therefore, a fusion protein with this part may have red color and the ability to be purified easily.
Documentation: