Composite
Part:BBa_K2382013
Designed by: SHAO-CHI LO Group: iGEM17_CSMU_NCHU_Taiwan (2017-10-26)
EAAAK+ RFP+Histag
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 606
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
This is a fragment of BBa_K2382010. We designed a restriction site, BamHI, before EAAAK rigid linker, so future iGEM teams could take advantage of this part to fuse their proteins with RFP as indicator.
Contents
Characterization of the EAAAK+ RFP+Histag
Improvement
This is an improvement of previous BioBrick Part BBa_E1010. It encodes a RFP that has
the same amino acids as BBa_E1010 and having a His-Tag at the end.
This part has codon optimized for E.coli. And we removed the barcode, which contains stop codon, from BBa_E1010. We hope this design could help other teams to utilize RFP in their fusion protein design and be easily purified.
References
BioBrick Part BBa_E1010
[edit]
Categories
Parameters
//cds/reporter/rfp
None |