Composite

Part:BBa_K2382013

Designed by: SHAO-CHI LO   Group: iGEM17_CSMU_NCHU_Taiwan   (2017-10-26)

EAAAK+ RFP+Histag


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 606
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This is a fragment of BBa_K2382010. We designed a restriction site, BamHI, before EAAAK rigid linker, so future iGEM teams could take advantage of this part to fuse their proteins with RFP as indicator.


Characterization of the EAAAK+ RFP+Histag

Improvement

This is an improvement of previous BioBrick Part BBa_E1010. It encodes a RFP that has the same amino acids as BBa_E1010 and having a His-Tag at the end.
This part has codon optimized for E.coli. And we removed the barcode, which contains stop codon, from BBa_E1010. We hope this design could help other teams to utilize RFP in their fusion protein design and be easily purified.

References

BioBrick Part BBa_E1010
[edit]
Categories
//cds/reporter/rfp
Parameters
None