Coding

Part:BBa_K2381009

Designed by: Anqi Zhou   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 17:38, 1 November 2017 by AnqiChou (Talk | contribs)


Conventional split dCas9-2

It is the first part of split dCas9 which is cut at the amino acid site 713 and 714.

References

Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None