Part:BBa_K2381009

Conventional split dCas9-2

It is the second part of split dCas9 which is cut at the amino acid site 713 and 714. This part is the from site 714 to site 1368.

References

Nihongaki, Yuta, et al. "Photoactivatable CRISPR-Cas9 for optogenetic genome editing.." Nature Biotechnology 33.7 (2015).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1239
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]