Composite

Part:BBa_K2448034

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)
Revision as of 11:03, 1 November 2017 by Brumake (Talk | contribs)


D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens

This is the original sequence of the D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens str. C58 (Atu4750, UniProt A9CH28, BBa_K2448022) equipped with a strong RBS (BBa_K2448020).

Usage and Biology

D-Psicose 3-epimerase (EC: 5.1.3.30) catalyses the reversible epimerization of D-fructose into D-psicose, D-psicose being the carbon-3 (C3) epimer of D-fructose.

T--Evry Paris-Saclay--EC 5.1.3.30.png

The A. tumefaciens Dpe is a 31.56 kDa protein of 289 amino acids.

This enzyme is highly specific for D-Psicose, but it is also able to catalyse with very low activity the epimerization of other D-ketoses (in the decreased substrate specificity) : D-fructose, D-tagatose, D-ribulose, D-xylulose and D-sorbose [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 208
    Illegal NgoMIV site found at 268
    Illegal NgoMIV site found at 619
    Illegal AgeI site found at 646
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 174


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Categories
Parameters
None