Part:BBa_K2448034

D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens

This is the original sequence of the D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens str. C58 (Atu4750, UniProt A9CH28, BBa_K2448022) equipped with a strong RBS (BBa_K2448020).

Usage and Biology

D-Psicose 3-epimerase (EC: 5.1.3.30) catalyses the reversible epimerization of D-fructose into D-psicose, D-psicose being the carbon-3 (C3) epimer of D-fructose.

The A. tumefaciens Dpe is a 31.56 kDa protein of 289 amino acids.

This enzyme is highly specific for D-Psicose, but it is also able to catalyse with very low activity the epimerization of other D-ketoses (in the decreased substrate specificity) : D-fructose, D-tagatose, D-ribulose, D-xylulose and D-sorbose [1]. For optimal activity, it requires cofactors such as Mn2+ or Co2+, although it has a low basal activity without ions. The native Dpe shows a tetrameric arrangement of 132 kDa, and each subunit has 289 amino acids with a molecular weight of 33 kDa. The topology of each subunit is a TIM-barrel fold with a cluster of eight β-strands surrounded by twelve α-helices [2].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 208
  Illegal NgoMIV site found at 268
  Illegal NgoMIV site found at 619
  Illegal AgeI site found at 646
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 174