Part:BBa_K2440014
miR-195 target sequence
It is the target sequence of miR-195, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-195 in an Ago2 dependent manner, that is, knockdown of miR-195 was achieved by transfecting cells with miRNA locker.
Hsa-miR-195 was firstly identified in lung, skin and kidney of mouse by cloning from mouse tissues.1
MicroRNA-195 has been reported as a cancer-related microRNA in many human cancers. Researchers find that miR-195 directly targets the fatty acid synthase enzyme and negatively regulates the expression of fatty acid synthase and finally demonstrated that MiR-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase.2
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.
Reference
1.New microRNAs from mouse and human. Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003).
2.MicroRNA-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase/Wnt signaling pathway.Xu Z, Li C, Qu H, Li H, Gu Q3, Xu J.
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