Part:BBa_K2440014

miR-195 target sequence

It is the target sequence of miR-195, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-195 in an Ago2 dependent manner, that is, knockdown of miR-195 was achieved by transfecting cells with miRNA locker.

Hsa-miR-195 was firstly identified in lung, skin and kidney of mouse by cloning from mouse tissues.¹

MicroRNA-195 has been reported as a cancer-related microRNA in many human cancers. Researchers find that miR-195 directly targets the fatty acid synthase enzyme and negatively regulates the expression of fatty acid synthase and finally demonstrated that MiR-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase.²

Sequence and Features

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.

Reference

1.New microRNAs from mouse and human. Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003).

2.MicroRNA-195 inhibits the proliferation and invasion of pancreatic cancer cells by targeting the fatty acid synthase/Wnt signaling pathway.Xu Z, Li C, Qu H, Li H, Gu Q, Xu J.