Coding

Part:BBa_K2374003

Designed by: Qian Zeng   Group: iGEM17_Tongji_China   (2017-10-20)
Revision as of 18:47, 31 October 2017 by Amang Z (Talk | contribs)


UAS-TH -> (fruit fly)

TH
Use in D.melanogaster
RFC standard RFC 10 compatible
Backbone pSB1C3
Submitted by [http://2017.igem.org/Team:Tongji_China Tongji_China 2017]

ple (Tyrosine 3-monooxygenase, TH)

ple (TH) is a rate-limiting enzyme in the dopamine’s synthesis, and it plays an important role in the physiology of adrenergic neurons.
Catalytic activity: L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin.
This subpathway is part of the pathway dopamine biosynthesis, which is itself part of catecholamine biosynthesis.


dopa


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 13
    Illegal PstI site found at 256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 13
    Illegal PstI site found at 256
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1540
    Illegal XhoI site found at 672
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 13
    Illegal PstI site found at 256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 13
    Illegal PstI site found at 256
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 918
    Illegal BsaI site found at 1884
    Illegal BsaI.rc site found at 636
    Illegal BsaI.rc site found at 1402
    Illegal BsaI.rc site found at 1551
[edit]
Categories
Parameters
None