DNA

Part:BBa_K2323013:Design

Designed by: Aurore Dupin   Group: iGEM17_Munich   (2017-10-29)
Revision as of 20:38, 29 October 2017 by Registry (Talk | contribs)

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Spacer between His tag and coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There's no special considerations here, other than the RBS needs to be correctly transcribed, and accessible for the ribosome. It should therefore be a few nucleotides after the first transcribed nucleotide of the mRNA


Source

random DNA sequence

References