Part:BBa_K2323013:Design

Spacer between His tag and coding sequence

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

There's no special considerations here. It's a spacer sequence. It should be a multiple of 3 nucleotides, to not shift the reading frame.

Source

random DNA sequence