Reporter

Part:BBa_K2375001

Designed by: Philip Leung, Merrick Pierson Smela, Sophie Vrba   Group: iGEM17_Minnesota   (2017-10-26)
Revision as of 14:15, 29 October 2017 by Piers199 (Talk | contribs)


mCherry Optimized, no internal RBS

mCherry was optimized for E. coli usage. Also, the internal RBS that had been causing problems was removed. To do this, residue 7 was mutated from Glu (GAG) to Ser (TCT). The part sequence was based on BBa_J06504

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None