Reporter
Part:BBa_K2375001
Designed by: Philip Leung, Merrick Pierson Smela, Sophie Vrba Group: iGEM17_Minnesota (2017-10-26)
mCherry Optimized, no internal RBS
mCherry was optimized for E. coli usage. Also, the internal RBS that had been causing problems was removed. To do this, residue 7 was mutated from Glu (GAG) to Ser (TCT). The part sequence was based on BBa_J06504.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |