Coding

Part:BBa_K2455005

Designed by: Jon Fugl   Group: iGEM17_UCopenhagen   (2017-10-27)
Revision as of 19:11, 28 October 2017 by Jon UCPH (Talk | contribs)


CPP-mTag BFP

DISCLAIMER: This biobrick carries and illegal EcoRI site partly in the end of the BFP and party in the beginning of the linker region.

This is an improved version of the mTag BFP (BBa_K592100) carrying an N-terminal nona-arginin tag, and a C-terminal hexa-histidine tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 741
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 741
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 741
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 741
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 741
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter
//collections/chromoprotein/uppsala
//function/reporter/fluorescence
Parameters
abs399 nm
colorBlue
directionForward
emit456
excite399
functionCell-penetrating, Fluorescent
proteinmTag BFP
tagC-terminal hexa-histidine